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藤井さんの論文がJ Anatomyに掲載

  • 比較解剖学的研究で哺乳類の気管支樹の基本モデルとして提案されている左右の気管支の対称性が、ヒトの胎児の気管支樹に当てはまるかどうかを検討
  • ヒト成人の気管支分岐構造の原型が胎児期に形成され、維持されることを実証
  • すべての肺葉気管支、B6、B8、B9、および B10 の亜分節気管支の形態および分岐位置は、遺伝的に決定される可能性がある
  • B10 の亜分節気管支以降の末梢枝では個々の胚間に共通の構造は見つからず、この領域での枝形成は遺伝的要因よりも環境的要因により影響されることが示唆された

Fujii S, Muranaka T, Matsubayashi J, Yamada S, Yoneyama A, Takakuwa T. Bronchial tree of the human embryo: Examination based on a mammalian model. J Anatomy 2024, 244, 159-169 http://doi.org/10.1111/joa.13946 .

The symmetry of right and left bronchi, proposed in a previous comparative anatomical study as the basic model of the mammalian bronchial tree, was examined to determine if it applied to the embryonic human bronchial tree. Imaging data of 41 human embryo specimens at Carnegie stage (CS) 16–23 (equivalent to 6–8 weeks after fertilization) belonging to the Kyoto collection were obtained using phase-contrast X-ray computed tomography. Three-dimensional bronchial trees were then reconstructed from these images. Bronchi branching from both main bronchi were labeled as dorsal, ventral, medial, or lateral systems based on the branching position with numbering starting cranially. The length from the tracheal bifurcation to the branching point of the labeled bronchus was measured, and the right-to-left ratio of the same labeled bronchus in both lungs was calculated. In both lungs, the human embryonic bronchial tree showed symmetry with an alternating pattern of dorsal and lateral systems up to segmental bronchus B9 as the basic shape, with a more peripheral variation. This pattern is similar to that described in adult human lungs. Bronchial length increased with the CS in all labeled bronchi, whereas the right-to-left ratio was constant at approximately 1.0. The data demonstrated that the prototype of the human adult bronchial branching structure is formed and maintained in the embryonic stage. The morphology and branching position of all lobar bronchi and B6, B8, B9, and the subsegmental bronchus of B10 may be genetically determined. On the other hand, no common structures between individual embryos were found in the peripheral branches after the subsegmental bronchus of B10, suggesting that branch formation in this region is influenced more by environmental factors than genetic factors.

福井さんの修士論文がJ Anatomyに掲載

福井さんの修士論文がJ Anatomyに受諾されました。ヒト胎児期初期の左房の形成を肺静脈のとりこみと左心耳の形成を中心に解析しました。

  • 23例の胚子期、19例の胎児期初期標本を選択した。
  • 高分解能イメージング(位相CTとMRI)から三次元心臓画像を再構成し、左心耳を含む肺静脈と左心房を形態学的および定量的に評価した。
  • 心臓の背側中膜結合が退縮したとき(CS18以降)、心膜折り返しの位置が2つの肺静脈(右肺静脈と左肺静脈)と4つの肺静脈でほぼ同時に決定された。
  • 左心房のひだが体部と静脈成分の接合部に存在することが確認された。
  • 左心房の肺静脈由来の静脈成分は成長に比例して増加することが示された。肺静脈成分と左心房体との接合部は徐々に目立たなくなったが、観察された初期胎児期の終わりにはまだ認識可能であった。

Fukui N, Toru KanahashiT, MatsubayashiJ, ImaiH, YoneyamaA, OtaniH, YamadaS, Takakuwa T. Morphogenesis of the pulmonary vein and left atrial appendage in human embryos and early fetuses. J Anatomy 2024, 244, 142-158, in press, https://doi.org/10.1111/joa.13941

Abstract

The left atrium wall has several origins, including the body, appendage, septum, atrial–ventricular canal, posterior wall, and venous component. Here, we describe the morphogenesis of left atrium based on high-resolution imaging (phase-contrast X-ray computed tomography and magnetic resonance imaging). Twenty-three human embryos and 19 fetuses were selected for this study. Three-dimensional cardiac images were reconstructed, and the pulmonary veins and left atrium, including the left atrial appendage, were evaluated morphologically and quantitatively. The positions of the pericardial reflections were used as landmarks for the border of the pericardial cavity. The common pulmonary vein was observed in three specimens at Carnegie stage 17–18. The pericardium was detected at the four pulmonary veins (left superior, left inferior, right superior, and right inferior pulmonary veins) at one specimen at Carnegie stage 18 and all larger specimens, except the four samples. Our results suggest that the position of the pericardial reflections was determined at two pulmonary veins (right and left pulmonary vein) and four pulmonary veins almost simultaneously when the dorsal mesocardial connection between the embryo and heart regressed. The magnetic resonance images and reconstructed heart cavity images confirmed that the left atrium folds were present at the junction between the body and venous component. Three-dimensional reconstruction showed that the four pulmonary veins entered the dorsal left atrium tangentially from the lateral to the medial direction. More specifically, the right pulmonary veins entered at a greater angle than the left pulmonary veins. The distance between the superior and inferior pulmonary veins was shorter than that between the left and right pulmonary veins. Three-dimensional reconstruction showed that the venous component increased proportionally with growth. No noticeable differences to discriminate between the right and left parts of the venous component emerged, while the junction between the venous component and body gradually became inconspicuous but was still recognizable by the end of the observed early fetal period. The left superior pulmonary vein had the smallest cross-sectional area and most flattened shape, whereas the other three were similar in area and shape. The left atrial appendage had a large volume in the center and extended to the periphery as a lobe-like structure. The left atrial appendage orifice increased in the area and tended to become flatter with growth. The whole left atrium volume^(1/3) increased almost proportionally with growth, parallel to the whole heart volume. This study provided a three-dimensional and quantitative description of the developmental process of left atrium, comprising the venous component and left atrial appendage formation, from the late embryonic to the early fetal stages.

第99回日本解剖学会学術集会近畿支部で発表しました

第99回日本解剖学会学術集会近畿支部で発表しました(11/18、京都)

松田幸樹、松林潤、金橋徹、山田重人、大谷浩、高桑徹也、ヒト胎児期における足関節周辺の三次元解析
中井尚一、松林潤、金橋徹、今井宏彦、山田重人、大谷浩、高桑徹也、ヒト胎児における主要血管径の左右差の比較検討 中井尚一
岩佐結生、金橋徹、今井宏彦、大谷浩、松林潤、山田重人、高桑徹也、MRIを用いたヒト胎児における腱画形成過程の解析

金橋徹、松林潤、今井宏彦、山田重人、大谷浩、高桑徹也、ヒト胎児骨盤の性差の検討

第3回医薬研究交流サロンで発表しました

9/27-29で行われる3回医薬研究交流サロンで発表しました

Toru Kanahashi, Hirohiko Imai, Hiroki Otani, Shigehito Yamada, Akio Yoneyama, Tetsuya Takakuwa; 拡散テンソル画像を用いたヒト胎児横隔膜の3次元的解析

Marie Ange Saizonou, Haruka Kitazawa, Toru Kanahashi, Shigehito Yamada, Tetsuya Takakuwa; Epithelial development of the urinary collecting system in the human embryo

中井尚一、松林潤、金橋徹、今井宏彦、山田重人、大谷浩、高桑徹也; ヒト胎児期初期から中期における血管の左右差の検討

岩佐結生, 金橋 徹, 松林 潤, 今井 宏彦, 大谷 浩, 山田 重人, 高桑 徹也; ヒト胎児における腱画形成過程の解析

須藤紗帆、金橋徹、今井宏彦、山田重人、高桑徹也;ヒト胚子期における舌筋の発生

八田桃佳、金橋徹,今井宏彦, 大谷浩,山田重人, 高桑 徹也;拡散テンソル画像を用いた水晶体線維細胞の配向性の検討

石田七彩、植田優生、松林潤、金橋徹、今井宏彦、山田重人、高桑徹也;ヒト中腸ループと腸間膜の経時的構造変化 ー生理的臍帯ヘルニア期から還納期ー

第17回日本臨床検査学教育学会で八田さんが 優秀発表賞(大学院)を受賞しました。

第17回日本臨床検査学教育学会学術大会 (2023.8.23-24, 天理)で発表しました。

八田桃佳、金橋徹今井宏彦山田重人、高桑 徹也、拡散テンソル画像を用いた水晶体線維細胞の配向性の検討

*八田さんが 優秀発表賞(大学院)を受賞しました。おめでとうございます!!

中井尚一、松林潤、金橋徹、今井宏彦、山田重人、大谷浩、高桑徹也、ヒト胎児期初期から中期の血管径変化より紐解く循環動態の検討

金橋 徹、伊吹謙太郎、山田 稔、榛葉旭恒、高桑徹也、寄生虫顕微鏡スライド標本のデジタル化に向けた取り組み

第63回日本先天異常学会で発表しました

第63回日本先天異常学会で発表しました (2023.7.28-30、つくば)

石田七彩、植田優生、松林潤、金橋徹、今井宏彦、山田重人、高桑徹也、生理的臍帯ヘルニア期のヒト中腸ループと腸間膜の経時的構造変化

岩佐結生、金橋徹、松林潤、今井宏彦、大谷 浩、高桑徹也、腹直筋の腱画形成過程

胚子期の股関節の肢位についての論文がPLoS Oneに掲載

胚子期の股関節の肢位についての論文がPLoS Oneに掲載されました。熊野くんが卒業研究で行ったデータに、胚子期の標本のデータを追加し解析しました。大腿骨(股関節)の肢位を正確に測定し、下肢の発達の時間軸を確立することを目的とした論文です。

  • CS19-23の胚子期157体と胎児(CRL:37.2-225mm)18体のMRI画像を対象とした。
  • 下肢と骨盤の選択された8つのランドマークの3次元座標を用いて大腿骨の肢位を計測。
  • 胚子期には、股関節の屈曲、外転、側転の3つの姿勢パラメータが互いに線形相関を示し、各段階の大腿骨姿勢は3次元的に一定で、成長に応じて緩やかで滑らかな変化を示すことが示唆された。

60. Takakuwa T, Saizonou MA, Fujii S, Kumano Y, Ishikawa A, Aoyama T, Imai H, Yamada S, Kanahashi T. Femoral posture during embryonic and early fetal development: An analysis using landmarks on the cartilaginous skeletons of ex vivo human specimens. PLOS one, 2023, 18(5): e0285190. https://doi.org/10.1371/journal.pone.0285190.

Abstract

The pre-axial border medially moves between the fetal and early postnatal periods, and the foot sole can be placed on the ground. Nonetheless, the precise timeline when this posture is achieved remains poorly understood. The hip joint is the most freely movable joint in the lower limbs and largely determines the lower-limb posture. The present study aimed to establish a timeline of lower-limb development using a precise measurement of femoral posture. Magnetic resonance images of 157 human embryonic samples (Carnegie stages [CS] 19–23) and 18 fetal samples (crown rump length: 37.2–225 mm) from the Kyoto Collection were obtained. Three-dimensional coordinates of eight selected landmarks in the lower limbs and pelvis were used to calculate the femoral posture. Hip flexion was approximately 14° at CS19 and gradually increased to approximately 65° at CS23; the flexion angle ranged from 90° to 120° during the fetal period. Hip joint abduction was approximately 78° at CS19 and gradually decreased to approximately 27° at CS23; the average angle was approximately 13° during the fetal period. Lateral rotation was greater than 90° at CS19 and CS21 and decreased to approximately 65° at CS23; the average angle was approximately 43° during the fetal period. During the embryonic period, three posture parameters (namely, flexion, abduction, and lateral rotation of the hip) were linearly correlated with each other, suggesting that the femoral posture at each stage was three-dimensionally constant and exhibited gradual and smooth change according to growth. During the fetal period, these parameters varied among individuals, with no obvious trend. Our study has merits in that lengths and angles were measured on anatomical landmarks of the skeletal system. Our obtained data may contribute to understanding development from anatomical aspects and provide valuable insights for clinical application.

22年度卒業式

22年度卒業式が行われました。久しぶりの対面での開催でした。

おめでとうございます