スクリーンショット 2014-01-18 16.19.10


Before we are born…

Many people – some scientists, others not – are interested in early human development because it is closely
related to the basic and important question: What are we and where have we come from?

Approximately 3-4% of newborn babies suffer from congenital anomalies (birth defects), which is a serious burden for patients and their families. Many congenital anomalies are assumed to be caused by the interaction of gene mutations and environmental factors, but the etiology and pathogenetic mechanisms remain to be clarified for most birth defects. In our laboratory, interdisciplinary research approaches are being undertaken to elucidate the causes and pathogenesis of birth defects and to attempt to identify some preventive measures.

3D analysis of human embryos and fetuses using digitized datasets from the Kyoto Collection

Three-dimensional (3D) analysis of the human embryonic and early-fetal period has been performed using digitized datasets obtained from the Kyoto Collection, in which the digital datasets play a primary role in research. Datasets include magnetic resonance imaging (MRI) acquired with 1.5 T, 2.35 T, and 7 T magnet systems, phase-contrast X-ray computed tomography (CT), and digitized histological serial sections. Large, high-resolution datasets covering a broad range of developmental periods obtained with various methods of acquisition are key elements for the studies. The digital data have gross merits that enabled us to develop various analysis. Digital data analysis accelerated the speed of morphological observations using precise and improved methods by providing a suitable plane for a morphometric analysis from staged human embryos. Morphometric data are useful for quantitatively evaluating and demonstrating the features of development and for screening abnormal samples, which may be suggestive in the pathogenesis of congenital malformations. Morphometric data are also valuable for comparing sonographic data in a process known as “sonoembryology.” The 3D coordinates of anatomical landmarks may be useful tools for analyzing the positional change of interesting landmarks and their relationships during development. Several dynamic events could be explained by differential growth using 3D coordinates. Moreover, 3D coordinates can be utilized in mathematical analysis as well as statistical analysis. The 3D analysis in our study may serve to provide accurate morphologic data, including the dynamics of embryonic structures related to developmental stages, which is required for insights into the dynamic and complex processes occurring during organogenesis

  1. Introduction
  2. Materials & Methods
  3. Results
  4. Discussion

■ Intestinal loop formation

■ Kyoto Collection

■ Embryo imaging

■ Publications


Tetsuya Takakuwa MD, PhD
Professor. Tetsuya Takakuwa MD, graduated from University of Tokyo, Faculty of Science in 1986, and graduated from Osaka University, Faculty of Medicine in 1990. He worked as an Acute Care Physician at Osaka University Hospital and Critical Care and Emergency center Iwate Medical collage for 8 years. During those years, he got an opportunity to study at Max-Plank Institute for Immuno-biology in Germany.   He took the role of Associate professor Department of Pathology, Osaka University Graduate School of Medicine in 2002, and received his PhD in 2005. He has become Professor of Kyoto University Graduate School of Medicine, Department of Human Health Science since 2008.

Human Health Science, Graduate School of Medicine, Kyoto University
606-8507 Sakyo-ku Shogoin Kawahara-cyo 53, Kyoto, Japan